Rhodnius prolixus Assembly and Gene Annotation

VectorBase The Rhodnius prolixus data and its display on Ensembl Genomes are made possible through a joint effort by the Ensembl Genomes group and VectorBase, a NIAID Bioinformatics Resource Center. The source data for this genome and other resources can be accessed via the VectorBase browser.

About Rhodnius prolixus

Rhodnius prolixus is a triatomine vector of the Chagas disease parasite, Trypanosoma cruzi, in South and Central America. Chagas disease is a chronic condition which affects 8 to 10 million people and is the cause of around 20,000 deaths per year. Triatomine insects release parasites in their faeces immediately after a blood meal. Infection occurs after the human host scratches the bite area, thus transferring parasites into the bite wound.

Picture credit (public domain): Dr. Erwin Huebner 2009

Assembly

Rhodnius prolixus was sequenced by the Washington University School of Medicine, Genome Sequencing Center (WUGSC) to a depth of 8.1x coverage, and assembled using the CABOG assembler. DNA was isolated from the colony at the Centers for Disease Control in Atlanta, USA. Assembly version RproC3, also known as Rhodnius_prolixus-3.0.3, is composed of 16,537 supercontigs.

Annotation

The annotation of the Rhodnius prolixus genome has been conducted by VectorBase. Genes were predicted using the Ensembl pipeline, which combines the results from ab initio predictions from Genscan and SNAP, alignments of dipteran and other protein sets, and R. prolixus ESTs. The geneset presented here (RproC3.2, August 2017) includes annotation by the Rhodnius community to improve and validate gene models and non-coding RNA genes from the Ensembl Genomes pipeline.

References

  1. Genetics and evolution of triatomines: from phylogeny to vector control.
    Gourbire S, Dorn P, Tripet F, Dumonteil E. 2012. Heredity. 108:190-202.
  2. The case for sequencing the genome of the blood-feeding hemipteran insect, Rhodnius prolixus.
    Huebner E, O'Donnell M, Lowenberger C, Steel C, Orchard I, Levin M, Garcia B, Esteban H, Oscar SD, Morales MM et al.. VectorBase: Genome project white paper.

More information

General information about this species can be found in Wikipedia.

Statistics

Summary

AssemblyRproC3, INSDC Assembly GCA_000181055.3, Apr 2015
Database version92.3
Base Pairs564,634,549
Golden Path Length706,824,083
Genebuild byVectorBase
Genebuild methodFull genebuild
Data sourceVectorBase

Gene counts

Coding genes15,071
Non coding genes623
Small non coding genes622
Long non coding genes1
Pseudogenes47
Gene transcripts15,755

Other

Genscan gene predictions18,055
Snap gene prediction65,399

About this species