Anopheles epiroticus (Mosquito, Epiroticus2) Assembly and Gene Annotation

About Anopheles epiroticus

The Anopheles sundaicus complex includes Anopheles epiroticus (formerly A. sundaicus A), which is a malaria vector located in Cambodia, peninsular Malaysia, Sarawak, Thailand and Vietnam.

Epiroticus2 strain

Sequencing was performed on wild collected individuals from Vietnam (Bac Lieu, October 2009), as a result, no colony exists. Mosquitoes were donned by Lien Tran and Martin Donnelly (Liverpool School of Tropical Medicine)

Source: VectorBase

AepiE1 assembly

This assembly was generated using 101 bp paired-end Illumina HiSeq2000 reads generated from two libraries: a 180 bp insert 'fragment' library and a 1.5 kb 'jump' library. Sequencing template for the fragment and jump libraries was derived from genomic DNA extracted from a single individual, which was preserved by freezing at -80C. Native genomic DNA was used for the fragment library and whole genome amplified DNA was used for the jump library. Reads were assembled at the Broad Institute using the ALLPATHS LG algorithm, with the Haploidify option enabled to address high allelic heterozygosity in the template.

AepiE1.8 gene set

Community annotation patch build for July 2019.