Anopheles maculatus (Mosquito, maculatus3) Assembly and Gene Annotation

About Anopheles maculatus

Range

Anopheles maculatus complex includes important malaria vectors distributed from the Indian subcontinent through Southeast Asia to Taiwan. Anopheles maculatus B is a vector member of this complex.

Habitats

Members of the Maculatus Group are typically found in or near hilly and mountainous areas. Larvae have been collected in a diverse number of permanent or semi-permanent bodies of clean water that are often exposed to direct sunlight, including ponds, lakes, swamps, ditches, wells, different types of pools (grassy, sandy, ground, flood, stream), margins along small, slow-flowing streams, gravel pits along stream margins, seepages, springs, rice fields, foot and wheel prints, and occasionally tree holes and bamboo stumps. More specific studies have shown that each species has a preferred habitat. For instance, larvae of An. willmori are found only along stream margins at altitudes between 990m and 1450m in northern Thailand, whereas larvae of An. pseudowillmori have been collected primarily in rice fields, stream margins, ponds, pits and wells. An. maculatus prefers to use pools of water formed on the banks of rivers and waterfalls most commonly shallow pools 5-15cm deep with clear water, mud substrate and emergent plants. This species also requires, or strongly prefers, open to partially shaded habitats. Habitats are commonly located at 100m to 400m from the nearest human settlement.

Resting and feeding preferences

In general, females are more strongly attracted to cattle than humans, but freely bite people both inside and outside houses. An. maculatus and An. sawadwongporni appear to be the least zoophilic of the species and exhibit early biting activity, peaking between 18:00 and 21:00. An. dispar and An. greeni exhibit strong exophagic and zoophilic behaviours. The combination of the early evening biting activity of these malaria vectors (particularly An. maculatus and An. sawadwongporni) and their zoophilic and exophilic tendencies indicates that they will be less affected by vector control methods based on indoor residual spraying and insecticide-treated bed nets. However, a strategy of creating a barrier using insecticide on vegetation near cattle or other animal hosts may provide significant in the control of these vectors.

Vectorial capacity

Members of the Maculatus Group are variously involved in malaria transmission. However, the vector role of each species is not precisely known and their vectorial capacity appears to vary depending on geographical location. An. maculatus has the widest distribution of all species of the group but it is only an important vector of human malarial parasites in hilly areas of eastern India, southern Thailand, peninsular Malaysia and south-central Java. Anopheles sawadwongporni is considered an important vector in Thailand and An. pseudowillmori is a secondary vector in northwestern Thailand along the Myanmar border. Anopheles willmori is one of the primary vectors in Nepal, but it is seldom collected in Thailand and does not appear to be involved in malaria transmission there. Anopheles dispar and An. greeniare regarded as secondary vectors in the Philippines. Anopheles notanandai, An. dravidicus and An. rampae are not known to be involved in malaria transmission.

This text was modified from Sinka ME et al. (2011) The dominant Anopheles vectors of human malaria in Asia-Pacific: occurrence data, distribution maps and bionomic précis Parasites & Vectors 4:89.

maculatus3 strain

These individuals were collected from Kuala Lumpur and sequencing was performed on preserved females donated by Lee Han Lim. No colony is available.

Source: VectorBase

AmacM1 assembly

This assembly was generated using 101 bp paired-end Illumina HiSeq2000 reads generated from two libraries: a 180 bp insert 'fragment' library and a 1.5 kb 'jump' library. Sequencing template for the fragment and jump libraries was derived from genomic DNA extracted from a single individual, which was preserved in ethanol. Native genomic DNA was used for the fragment library and whole genome amplified DNA was used for the jump library. Reads were assembled at the Broad Institute using the ALLPATHS LG algorithm, with the Haploidify option enabled to address high allelic heterozygosity in the template.

AmacM1.6 gene set

Community annotation patch build for July 2019.

References

1. The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic prcis.
   Sinka ME, Bangs MJ, Manguin S, Chareonviriyaphap T, Patil AP, Temperley WH, Gething PW, Elyazar IR, Kabaria CW, Harbach RE et al. 2011. Parasit Vectors. 4

Picture credit: VectorBase.org