Anopheles quadriannulatus (Mosquito, SANGWE) Assembly and Gene Annotation

About Anopheles quadriannulatus

Anopheles quadriannulatus A belongs to the Anopheles gambiae species complex, which consists of at least seven species, it is found in southern Africa and is not considered to be a malaria vector.

Strain SANGWE (aka SANGQUA)

Originally isolated in South Africa the colony subsequently undergone rounds of isofemale selection prior to genome sequencing. The SANGWE strain is a synonym for the SANGQUA strain (this strain is available from BEI resources).

Source: VectorBase

Picture credit: James Gathany, CDC Public domain via Wikimedia Commons (Image source)

AquaS1 assembly

This assembly was generated using 101 bp paired-end Illumina HiSeq2000 reads generated from three libraries: a 180 bp insert 'fragment' library, a 1.5 kb 'jump' library, and a 38 kb 'fosill' library. Sequencing template for the fragment and jump libraries was derived from genomic DNA extracted from a single individual, which was preserved by freezing at -80C. Native genomic DNA was used for the fragment library and whole genome amplified DNA was used for the jump library. Template for the fosill library was generated from a pooled extraction of many individuals. Reads were assembled at the Broad Institute using the ALLPATHS LG algorithm, with the Haploidify option enabled to address high allelic heterozygosity in the template.

AquaS1.12 gene set

Community annotation patch build for July 2019