Culex quinquefasciatus (Southern house mosquito, Johannesburg) Assembly and Gene Annotation

About Culex quinquefasciatus

Culex quinquefasciatus, the Southern House Mosquito, is the vector responsible for transmission of filariasis and a number of viruses including West Nile virus, St. Louis encephalitis and Japanese encephalitis.

Picture credit (public domain): James Gathany (CDC) 2001

Assembly

The Culex quinquefasciatus Johannesburg strain genome sequence is a joint effort between the Broad Institute and the J. Craig Venter Institute (JCVI). Assembly of 8x shotgun coverage was performed using the Broad's whole genome assembly package ARACHNE. The assembly presented here (CpipJ2, April 2014) consists of 3,171 supercontigs and a mitochondrial chromosome, totalling 580 Mb.

Annotation

The annotation of the C. quinquefasciatus genome is a collaboration between VectorBase, JCVI and the Broad Institute. Each group generated a set of gene predictions which were merged into a single canonical set [1]. The gene set currently shown (CpipJ2.5, July 2019) is an improved version of the initial set, with integration of community manual annotations and non-coding RNA genes from the Ensembl Genomes pipeline.

References

1. Sequencing of Culex quinquefasciatus establishes a platform for mosquito comparative genomics.
   Arensburger P, Megy K, Waterhouse RM, Abrudan J, Amedeo P, Antelo B, Bartholomay L, Bidwell S, Caler E, Camara F et al. 2010. Science. 330:86-88.